The coiled-coil domain regulates oligomerization of matrilins. A. Schematic diagram of Construct 1: MINI-MATN3; and Construct 2: MINI-MATN3_1CC. B. Western blot analysis of conditioned medium collected from Cos cells transfected with Construct 1, or 2 using antiserum against the V5 tag. Reducing conditions are indicated. Molecular weights of the MINI-MATN3 oligomers are indicated on the left, while those of MINI-MATN3_1CC are indicated on the right.