Site-specific analysis of gene expression in early osteoarthritis using the Pond-Nuki model in dogs

Table 1 Primer sets used for Real-Time PCR analysis

Gene	Orientation	Primer Sequence	Amplicon Size	Melt Temp
GAPDH	FOR	GTGACTTCAACAGTGACACC	152	84.7
	RC	CCTTGGAGGCCATGTAGACC
Aggrecan	FOR	ATCGAAGGGGACTTCCGCTG	106	84.5
	RC	ATCACCACACAGTCCTCTCCG
COL 2	FOR	GGCCTGTCTGCTTCTTGTAA	197	83.3
	RC	ATCAGGTCAGGTCAGCCATT
COL 1	FOR	TGCACGAGTCACACTGGAGC	124	85.5
	RC	ATGCCGAATTCCTGGTCTGG
TIMP 1	FOR	GCAGAAGTCAACCAGACCGA	311	86.2
	RC	GCAAGTATCCGCAGACGCTC
TIMP 2	FOR	AACGGCAAGATGCACATCAC	142	85.5
	RC	ATATAGCACGGGATCATGGG
INOS	FOR	GCTATGCTGGCTACCAGATG	139	88.3
	RC	ATCAGCCTGCAGCACCAGAG
COX-2	FOR	ACACTCTACCACTGGCATCC	196	83.5
	RC	GCTACTTGTTGTACTGCAGC
MMP-1	FOR	CCTAGAACCGTGAAGAGCAT	150	80
	RC	CAGGAAAGTCAGCTGCTATC
MMP 3	FOR	ATGGCATCCAGTCCCTGTAT	161	86.5
	RC	AAAGAACAGGAACTCTCCCC
MMP 13	FOR	TCTGGTCTTCTGGCTCATGC	141	82.7
	RC	GGTCAAGACCTAAGGAGTGG
ADAMTS4	FOR	CATCACTGAGTTCCTGGACA	106	84.5
	RC	CGATCAGCGTCATAGTCCTT
ADAMTS5	FOR	TGACTTCTTGCATGGCATGG	120	81.5
	RC	CTGGCATGGCTGGTGACTGA

Canine primer sets used for real-time PCR analysis. The annealing temperature used for all analysis was 57°C. The melt temperature for the amplicon was obtained from the Rotorgene software and is indicative of a specific PCR reaction.

ISSN: 1749-799X