From: Site-specific analysis of gene expression in early osteoarthritis using the Pond-Nuki model in dogs
Gene | Orientation | Primer Sequence | Amplicon Size | Melt Temp |
---|---|---|---|---|
GAPDH | FOR | GTGACTTCAACAGTGACACC | 152 | 84.7 |
RC | CCTTGGAGGCCATGTAGACC | |||
Aggrecan | FOR | ATCGAAGGGGACTTCCGCTG | 106 | 84.5 |
RC | ATCACCACACAGTCCTCTCCG | |||
COL 2 | FOR | GGCCTGTCTGCTTCTTGTAA | 197 | 83.3 |
RC | ATCAGGTCAGGTCAGCCATT | |||
COL 1 | FOR | TGCACGAGTCACACTGGAGC | 124 | 85.5 |
RC | ATGCCGAATTCCTGGTCTGG | |||
TIMP 1 | FOR | GCAGAAGTCAACCAGACCGA | 311 | 86.2 |
RC | GCAAGTATCCGCAGACGCTC | |||
TIMP 2 | FOR | AACGGCAAGATGCACATCAC | 142 | 85.5 |
RC | ATATAGCACGGGATCATGGG | |||
INOS | FOR | GCTATGCTGGCTACCAGATG | 139 | 88.3 |
RC | ATCAGCCTGCAGCACCAGAG | |||
COX-2 | FOR | ACACTCTACCACTGGCATCC | 196 | 83.5 |
RC | GCTACTTGTTGTACTGCAGC | |||
MMP-1 | FOR | CCTAGAACCGTGAAGAGCAT | 150 | 80 |
RC | CAGGAAAGTCAGCTGCTATC | |||
MMP 3 | FOR | ATGGCATCCAGTCCCTGTAT | 161 | 86.5 |
RC | AAAGAACAGGAACTCTCCCC | |||
MMP 13 | FOR | TCTGGTCTTCTGGCTCATGC | 141 | 82.7 |
RC | GGTCAAGACCTAAGGAGTGG | |||
ADAMTS4 | FOR | CATCACTGAGTTCCTGGACA | 106 | 84.5 |
RC | CGATCAGCGTCATAGTCCTT | |||
ADAMTS5 | FOR | TGACTTCTTGCATGGCATGG | 120 | 81.5 |
RC | CTGGCATGGCTGGTGACTGA |