Fig. 3From: CircPRKD3/miR-6783-3p responds to mechanical force to facilitate the osteogenesis of stretched periodontal ligament stem cellsRegulation of circPRKD3 affected osteogenesis of mechanically stimulated PDLSCs. (A) Schematic diagram of virus construction. Immunofluorescence staining (B) and qRT-PCR verification (C) of transfection efficiency in sh-NC and sh-circPRKD3 groups. (D-E) The mRNA expression level of ALP and RUNX2 in mechanically stimulated sh-NC and sh-circPRKD3 groups after transfection for 24 h. (F-H) The protein level of ALP and RUNX2 and the quantitative data analyzed by image J in mechanically stimulated sh-NC groups and sh-circPRKD3 groups after transfection for 24 h. Full-length blots were presented in Supplementary Fig. 2 of additional file 1. (I) Schematic diagram of plasmids construction. (J) The head-to‐tail splicing of circPRKD3 as a qRT‐PCR product was verified by Sanger sequencing. (K) Pure E. coli containing circPRKD3-overexpressed plasmids were filtered out by ampicillin. Verification of transfection efficiency in 293T (L) and PDLSCs (M) was assayed by qRT‐PCR. (N-O) The mRNA expression level of ALP and RUNX2 in mechanically stimulated pLC5‐ciR-NC groups and pLC5‐circPRKD3 groups after transfection for 24 h. (P-R) The protein level of ALP and RUNX2 and the quantitative data analyzed by image J in mechanically stimulated pLC5‐ciR-NC groups and pLC5‐circPRKD3 groups after transfection for 24 h. Full-length blots were presented in Supplementary Fig. 3 of additional file 1. Data are presented as mean ± SD of three independent experiments. (n.s., no significance; **p < 0.01; ****p < 0.0001)Back to article page