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Fig. 1 | Journal of Orthopaedic Surgery and Research

Fig. 1

From: Exosomes derived from M2 macrophages prevent steroid-induced osteonecrosis of the femoral head by modulating inflammation, promoting bone formation and inhibiting bone resorption

Fig. 1

Identification of M2 macrophages and exosomes. (A) Flow cytometry analysis of CD206(M2 marker) in PBS-treated macrophages. (B) Flow cytometry analysis of CD206(M2 marker) in IL-4-treated macrophages. (C-E) Expression levels of Arg-1, CD206 and IL-10(M2-specific markers) in PBS-treated or IL-4-treated cells measured by qRT-PCR. (F) Morphology of exosomes derived from M2 macrophages observed by TEM. (G) Size distribution of exosomes detected by nanoparticle tracking analysis. (H) Detection of exosome surface markers by Western blotting. The data are presented as the means ± SDs; N = 5; *p < 0.05

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X