Fig. 4
From: KLF5 promotes the ossification process of ligamentum flavum by transcriptionally activating CX43
CX43 is a direct transcriptional target of KLF5. (A) The transcriptional factor KLF5 binding motif from the JASPAR database. (B) The ChIP-PCR assay verified the direct binding of KLF5 and CX43 promoter in OLF fibroblasts. (C) Dual-luciferase reporter assays on the wild-type and mutated CX43 promoters. (D) Effect of si-KLF5 on mRNA level of CX43 was measured using RT-qPCR. (E) Effect of si-KLF5 on the protein level of CX43 was measured using western blot. *p < 0.05, **p < 0.01, ***p < 0.001