Fig. 8

FNDC5 modulated ferroptosis by the eIF2α-ATF4-CHOP pathway. (A, B) The cells were treated with HG with or without FNDC5 overexpression. Protein levels of eif2a, ATF4, and CHOP. *P < 0.05, **P < 0.01, ***P < 0.001 versus the con group. ^#P < 0.05, ^##P < 0.01 versus the HG group. The cells were treated with HG with or without GSK. (C, D) Protein levels of GPX4, SLC7A11, and FTH. (E) Representative images of the DCFH-DA staining (scale: 100 μm) and C11-BODIPY staining (scale bar: 100 μm) images. Red images represented nonoxidized lipids, while green images represented oxidized lipids. (F) GSH content and (G) MDA levels. **P < 0.01, ***P < 0.001 versus the con group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.05 versus the HG group