Fig. 6

FNDC5 overexpression improved ferroptosis in HG-induced MC3T3-C1 cells. MC3T3-E1 cells were cultured with various glucose concentrations (5.5, 11, 22, or 33 mM). (A) Relative mRNA level of FNDC5. (B, C) Protein level of FNDC5. ***P < 0.001 versus the 5.5 mm group. The cells were treated with HG with or without FNDC5 overexpression. (D, E) Protein levels of FNDC5, GPX4, SLC7A11, and FTH. (F) Representative images of the DCFH-DA staining (scale: 100 μm), and C11-BODIPY staining (scale bar: 100 μm). Red images represented nonoxidized lipids, while green images represented oxidized lipids. (G) GSH content and (H) MDA levels were measured. (I) Representative images of TEM (scale bar: 2.0 μm, 0.5 μm). Red arrowheads point to the mitochondria. OE-NC means negative control, OE-FNDC5 means overexpression of FNDC5. ***P < 0.001 versus the con group, ^##P < 0.01, ^###P < 0.001 versus the HG group