Table 1 Primer sequences used for qRT-PCR
From: Circ_0044235 regulates the development of osteoarthritis by the modulation of miR-375/PIK3R3 axis
Name | Primers for PCR | Product size | |
|---|---|---|---|
hsa_circ_0044235 | Forward | TGAGTTTGGTGATTCAGCTTGC | 152 bp |
Reverse | AACAAGGCTTCTTCTGAGTGT | ||
CDC27 | Forward | CAGTCTGTTGCCAGAATCGG | 166 bp |
Reverse | GTGCGTTTGGGGGAGATGTA | ||
miR-375 | Forward | GCGTTTGTTCGTTCGGCTC | 66 bp |
Reverse | AGTGCAGGGTCCGAGGTATT | ||
miR-1200 | Forward | GCGCTCCTGAGCCATTCTG | 66 bp |
Reverse | AGTGCAGGGTCCGAGGTATT | ||
miR-335 | Forward | CGCGTCAAGAGCAATAACGAA | 67 bp |
Reverse | AGTGCAGGGTCCGAGGTATT | ||
miR-338-5p | Forward | CGCGAACAATATCCTGGTGC | 66 bp |
Reverse | AGTGCAGGGTCCGAGGTATT | ||
miR-498 | Forward | TTCAAGCCAGGGGGCG | 67 bp |
Reverse | AGTGCAGGGTCCGAGGTATT | ||
miR-507 | Forward | CGCGTTTTGCACCTTTTGG | 65 bp |
Reverse | AGTGCAGGGTCCGAGGTATT | ||
miR-557 | Forward | TTTGCACGGGTGGGCC | 67 bp |
Reverse | AGTGCAGGGTCCGAGGTATT | ||
miR-574-5p | Forward | CGCGTGAGTGTGTGTGTGTGA | 67 bp |
Reverse | AGTGCAGGGTCCGAGGTATT | ||
miR-892a | Forward | CGCGCACTGTGTCCTTTCT | 65 bp |
Reverse | AGTGCAGGGTCCGAGGTATT | ||
PIK3R3 | Forward | CGGTCGGGTTGGTTCTTACA | 165 bp |
Reverse | CTGGTCTGCAGAGAGCGAAT | ||
GAPDH | Forward | GACAGTCAGCCGCATCTTCT | 104 bp |
Reverse | GCGCCCAATACGACCAAATC | ||
U6 | Forward | CTCGCTTCGGCAGCACA | 94 bp |
Reverse | AACGCTTCACGAATTTGCGT |