Fig. 6
From: Circ_0044235 regulates the development of osteoarthritis by the modulation of miR-375/PIK3R3 axis
Down-regulation of PIK3R3 partially restored the effect of anti-miR-375 in IL-1β-induced CHON-001 cell injury. (A) The knockdown efficiency of PIK3R3 was measured by western blot. (B) Relative PIK3R3 protein expression in each group was detected by western blot. (C and D) CCK-8 assay and flow cytometry analysis assay were conducted for the viability and apoptosis of CHON-001 cells. (E) The protein levels of Bax, Bcl-2 and cleaved-caspase-3 in CHON-001 cells were measured via western blot assay. (F) ELISA kits were used for the concentrations of TNF-α and IL-6 in CHON-001 cells. (G) Western blot assay was employed for the protein levels of MMP-13, Collagen II and Aggrecan in CHON-001 cells. *P < 0.05