Fig. 5
From: Circ_0044235 regulates the development of osteoarthritis by the modulation of miR-375/PIK3R3 axis
PIK3R3 was a target gene for miR-375. (A) The predicted binding sites between PIK3R3 and miR-375. (B and C) The interaction between miR-375 and PIK3R3 was analyzed by dual-luciferase reporter and RIP assays. (D and E) Relative miR-375 expression and PIK3R3 protein expression in anti-miR-NC or anti-miR-375-transfected CHON-001 cells was analyzed by qRT-PCR and western blot. (F and G) The mRNA and protein levels of PIK3R3 in OA cartilage tissues were determined by qRT-PCR assay and western blot assay, respectively. (H and I) The mRNA and protein levels of PIK3R3 in IL-1β-treated CHON-001 cells was measured by qRT-PCR assay and western blot assay. (J and K) The levels of PIK3R3 mRNA and protein in vector, oe-circ_0044235, oe-circ_0044235 + miR-NC or oe-circ_0044235 + miR-375 treated CHON-001 cells were measured by qRT-PCR assay and western blot assay, respectively. *P < 0.05