Fig. 3
From: Circ_0044235 regulates the development of osteoarthritis by the modulation of miR-375/PIK3R3 axis
Circ_0044235 directly targeted miR-375. (A) The circ_0044235 level was evaluated by qRT-PCR assay. (B) The expression levels of related miRNAs were detected by qRT-PCR. (C) The predicted binding sites between circ_0044235 and miR-375. (D) Relative miR-375 expression was determined in miR-NC or miR-375 mimic-transfected CHON-001 cells by qRT-PCR. (E) The luciferase activity in CHON-001 cells co-transfected with miR-NC/miR-375 and circ_0044235 WT/circ_0044235 MUT was measured by dual-luciferase reporter assay. (F) The enrichment of circ_0044235 and miR-375 was determined by RIP assay. (G and H) The expression level of miR-375 in OA cartilage tissues and IL-1β-stimulated CHON-001 cells was examined by qRT-PCR assay. *P < 0.05