Fig. 2
From: Circ_0044235 regulates the development of osteoarthritis by the modulation of miR-375/PIK3R3 axis
High expression of circ_0044235 reversed IL-1β-induced cell viability, apoptosis, inflammation, and ECM degradation in CHON-001 cells. (A) Relative circ_0044235 expression was determined by qRT-PCR in vector and oe-circ_0044235 group. (B) The expression level of circ_00442235 in IL-1β, IL-1β + vector, or IL-1β + oe-circ_0044235 treated CHON-001 cells or untreated cells (control) was determined by qRT-PCR assay. (C) Cell viability was evaluated by CCK-8 assay. (D) Cell apoptosis was examined by flow cytometry assay. (E) Relative expression of Bax, Bcl-2 and cleaved-caspase-3 was analyzed by western blot. (F) Secretion of inflammatory factor (TNF-α and IL-6) was detected by ELISA assay. (G) The protein expression of MMP-13, Collagen II and Aggrecan was estimated by western blot. *P < 0.05