Fig. 4
From: CYLD alleviates NLRP3 inflammasome-mediated pyroptosis in osteoporosis by deubiquitinating WNK1
CYLD enhanced the protein stability by deubiquitination of WNK1
(A-B) Expression of WNK1 in CYLD overexpressed OVX-BMSCs was assessed by qRT-PCR and western blot. (C) Co-IP validated the binding relationship between CYLD and WNK1 in 293T cells. (D) OVX-BMSCs transfected with NC or CYLD overexpression vectors were incubated with 50 ng/ml CHX for 0, 6, 12 h. Then, western blot was adopted to measure WNK1 level. (E) In vitro detection of WNK1 protein ubiquitination. Values were expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001