Fig. 2
From: CYLD alleviates NLRP3 inflammasome-mediated pyroptosis in osteoporosis by deubiquitinating WNK1
Overexpression of CYLD or WNK1 promoted osteogenic differentiation of OVX-BMSCs
BMSCs were isolated from sham mice and OVX mice, and named as sham-BMSCs and OVX-BMSCs, respectively. (A-B) qRT-PCR and Western blot was used to evaluate CYLD and WNK1 expression in sham-BMSCs and OVX-BMSCs. (C) qRT-PCR was utilized to test the transfection efficiency of CYLD or WNK1 overexpression vectors in OVX-BMSCs. (D-E) ARS and ALP staining was performed to detect osteogenic differentiation of OVX-BMSCs after CYLD or WNK1 overexpression. (F-G) qRT-PCR and Western blot was conducted to measure the levels of osteogenesis-related markers in cells with CYLD or WNK1 overexpression. Data were represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001