Fig. 1
From: CYLD alleviates NLRP3 inflammasome-mediated pyroptosis in osteoporosis by deubiquitinating WNK1
CYLD and WNK1 were decreased in OP mouse model
C57BL/6J mice were used for establishing an OP mouse model by OVX method. After experiments, femurs and tibias were collected. (A-D) Bone parameters including BMD, BV/TV, Tb.N and Tb.Th were measured. (E-F) HE and Masson staining was used to analyze the pathological changes of femurs and tibias. (G) qRT-PCR was adopted to measure the expressions of osteogenesis-related molecules (RUNX2, OCN, OPN). (H) Immunohistochemical staining was utilized to evaluate the expression of NLRP3. (I) Western blot was conducted to detect the levels of CYLD, WNK1, NLRP3, ASC, IL-1β and caspase1. Values are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001