Fig. 3

CASP3 as a potential target of miR-98-5p. A Sequence alignment of a putative miR-98-5p binding site within the 3′-UTR of CASP3 mRNA was shown. B CHON-001 cells were co-transfected with miR-98-5p mimics or miR-NC and luciferase reporter constructs of the wild-type CASP3 3′-UTR (WT 3′-UTR) or the mutated CASP3 3′-UTR (MUT 3′-UTR). Luciferase activity was measured after transfection. **p < 0.01 indicates miR-98-5p mimics versus miR-NC; The CASP3 mRNA (C) and protein (D) levels were measured in IL-1β-stimulated CHON-001 cells. All data are shown as means ± SD of three independent experiments. ***p < 0.001 indicates IL-1β versus Control; ##p < 0.01 and ###p < 0.001 indicate IL-1β + miR-98-5p mimics versus IL-1β