Fig. 7
From: Several first-line anti-hypertensives act on fibrosarcoma progression and PD1ab blockade therapy
HCTZ significantly promoted human HT1080 fibrosarcoma proliferation, migration, and invasion, accelerating tumor growth in vivo. A, B The CCK8 detection and colony formation assays were used to analyze the effect of HCTZ on the proliferative abilities of HT1080 cells. C The transwell assay were used to detect the effect of HCTZ on the migrative abilities of HT1080 cells. Representative photographs as well as quantification are demonstrated. Scale bars, 50 μm. D The effect of HCTZ on the migrative abilities of HT1080 cells was examined by cell wound healing assay. Representative photographs as well as quantification are demonstrated.Scale bars, 100 μm. E–G The effect of HCTZ on tumor growth in vivo was detected by xenograft growth assay (E). Tumor mass (F) and weight (G) were indicated (n = 6)