Fig. 4
From: Several first-line anti-hypertensives act on fibrosarcoma progression and PD1ab blockade therapy
Furosemide showed no effect on fibrosarcoma growth but weakened tumor immune responses to PD1ab. A CCK8 experiment was used to detect the proliferation ability of MCA-205 after furosemide treatment. B Elisa kit was used to detect the VEGF production after furosemide treatment. C A double therapy (furosemide and PD1ab) or monotherapies (furosemide or PD1ab) were used for curing MCA-205-neoplasms mice. D–F Tumor mass (D) and weight (E) from MCA-205-neoplasms mice (n = 10) were demonstrated. (F) Survival of mice (n = 10) was monitored biweekly after treatment. G–H The level of CD8+ T cells infiltration after treatment. Scale bars, 20 μm