Fig. 3

Losartan showed different effects on fibrosarcoma in vitro and in vivo. A CCK8 experiment was used to detect the proliferation ability of MCA-205 after losartan treatment. B Elisa kit was used to detect the VEGF production after losartan treatment. C A double therapy (losartan and PD1ab) or monotherapies (losartan or PD1ab) were used for curing MCA-205-neoplasms mice. D–F Tumor mass (D) and weight (E) from MCA-205-neoplasms mice (n = 10) were demonstrated. F Survival of mice (n = 10) was monitored biweekly after treatment. G–H The level of CD8⁺ T cells infiltration after therapy after treatment. Scale bars, 20 μm