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Fig. 6 | Journal of Orthopaedic Surgery and Research

Fig. 6

From: Periostin/Bone Morphogenetic Protein 1 axis axis regulates proliferation and osteogenic differentiation of sutured mesenchymal stem cells and affects coronal suture closure in the TWIST1+/− mouse model of craniosynostosis

Fig. 6

Periostin/BMP1 axis regulates SMSC proliferation and osteogenesis. pcDNA 3.1-Periostin and pcDNA 3.1-BMP1 were co-transfected into the SMSCs of the TWIST1+/− group. A Western blot assays were conducted to measure BMP1 protein expression in the SMSCs across the groups. B Colony formation assays were performed to evaluate the proliferative capacity of SMSCs. C EdU assays were utilized to further quantify the proliferation of SMSCs. D Wound healing assays were carried out to assess the wound closure rate of SMSCs. E ALP staining was employed to determine the ALP activity in SMSCs. F ARS staining was used to observe calcium deposition in SMSCs. G Western blot analysis was conducted to measure the expression levels of Ki-67, RUNX2, and OPN in SMSCs. Data were represented as mean ± SD (N = 3). *P < 0.05

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X