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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: Periostin/Bone Morphogenetic Protein 1 axis axis regulates proliferation and osteogenic differentiation of sutured mesenchymal stem cells and affects coronal suture closure in the TWIST1+/− mouse model of craniosynostosis

Fig. 2

Periostin effectively inhibits SMSC proliferation and osteogenic differentiation. A SMSCs were isolated from cranial suture bone tissues of both TWIST1+/− and TWIST1+/+ mice and identified by flow cytometry as CD44 and CD105 positive. TWIST1+/− SMSCs were transfected with pcDNA 3.1-Periostin. B Western blot assays were performed to assess Periostin protein expression in each group of SMSCs. C Colony formation assay was conducted to evaluate the proliferative capacity of SMSCs. D EdU assay was used to further quantify SMSC proliferation. E Wound healing assays were carried out to measure the wound closure rate of SMSCs. F ALP staining was used to determine ALP activity in SMSCs. G ARS staining was employed to observe calcium deposition in SMSCs. H Western blot analysis was conducted to measure the expression levels of Ki-67, RUNX2, and OPN in SMSCs. Data were presented as mean ± SD (N = 3). *P < 0.05

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X