Fig. 6

PDK4 inhibits IL-1β-induced chondrocyte apoptosis, inflammation, and ECM degradation by promoting the PPAR pathway. A IL-1β-induced chondrocyte viability was assessed by CCK-8 after PDK4 overexpression and GW9662 treatment. B Flow cytometry was performed to evaluate apoptosis in IL-1β-treated chondrocytes in four groups. C TNF-α, IL-8, and IL-6 levels were measured using ELISA in IL-1β-induced chondrocytes. D Western blot analysis was used to measure the expression of proteins linked to ECM degradation, including as MMP-3, MMP-13, and ADAMTS-4 after PDK4 overexpression and GW9662 treatment in IL-1β-induced chondrocytes. **P < 0.01, ***P < 0.001 versus IL-1β + ov-NC group; ^##P < 0.01, ^###P < 0.001 versus IL-1β + ov-PDK4 group