Table 1 qRT-PCR primer sequence and cycling conditions
From: Inhibition of HOXD11 promotes cartilage degradation and induces osteoarthritis development
Gene | NCBI Gene ID | GenBank Accession | Primer Sequence (Forward/Reverse)5′ → 3′ | TM (℃) | Amplicon Size (bp) |
|---|---|---|---|---|---|
SOX9 | 6662 | NM_000346 | AGCGAACGCACATCAAGAC | 60.7 | 85 |
CTGTAGGCGATCTGTTGGGG | 62 | ||||
COL2A1 | 1280 | NM_001844 | TGGACGATCAGGCGAAACC | 62 | 244 |
GCTGCGGATGCTCTCAATCT | 62.4 | ||||
HOXD9 | 3235 | NM_014213 | GGACTCGCTTATAGGCCATGA | 61.1 | 141 |
GCAAAACTACACGAGGCGAA | 60.9 | ||||
HOXD10 | 3236 | NM_002148 | GACATGGGGACCTATGGAATGC | 62.5 | 129 |
CGGATCTGTCCAACTGTCTACT | 60.9 | ||||
HOXD11 | 3237 | NM_021192 | TCGACCAGTTCTACGAGGCA | 62.5 | 325 |
AAAAACTCGCGTTCCAGTTCG | 61.7 | ||||
HOXD12 | 3238 | NM_021193 | CAGTCGCCAGACTCTTTCTACT | 61.1 | 235 |
GCTCTTCGGGTCCGTCTTT | 61.7 | ||||
HOXD13 | 3239 | NM_000523 | CTTCGGCAACGGCTACTACAG | 62.8 | 124 |
TGACACGTCCATGTACTTCTCC | 61.4 | ||||
MMP3 | 4314 | NM_002422 | AGTCTTCCAATCCTACTGTTGCT | 61 | 226 |
TCCCCGTCACCTCCAATCC | 63 | ||||
MMP13 | 4322 | NM_002427 | ACTGAGAGGCTCCGAGAAATG | 61.3 | 103 |
GAACCCCGCATCTTGGCTT | 62.7 | ||||
ADAMTS4 | 9507 | NM_005099 | GAGGAGGAGATCGTGTTTCCA | 60.9 | 118 |
CCAGCTCTAGTAGCAGCGTC | 61.8 | ||||
ADAMTS5 | 11,096 | NM_007038 | GAACATCGACCAACTCTACTCCG | 62.3 | 107 |
CAATGCCCACCGAACCATCT | 62.5 | ||||
GAPDH | 2597 | NM_001256799 | GGAGCGAGATCCCTCCAAAAT | 61.6 | 197 |
GGCTGTTGTCATACTTCTCATGG | 60.9 |