Fig. 4

Immunohistochemical and histological staining. A Differences in distal femur hematoxylin–eosin, OCN, and TRAP staining of the distal femur among groups. Red arrows indicate positive osteoblast and osteoclast, respectively. B The density of OCN and TRAP positive cells on the bone surface was quantified as cells per millimeter of bone perimeter (/B.Pm). Data were presented as means and standard deviations, and *P < 0.05 was used to compare the study to a control group, and #P 0.05 was used to compare the KD and KD + icariin groups