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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: MicroRNA-877-5p promotes osteoblast differentiation by targeting EIF4G2 expression

Fig. 4

Suppressive effects of EIF4G2 on osteoblast differentiation. (A) RT-qPCR and Western blot to verify the successful transfection of si-EIF4G2 or oe-EIF4G2; (B/C) alizarin red staining to detect the mineralization of MC3T3-E1 cells; (D) ALP activity assay; (E–G) RT-qPCR detection of ALP, COLIA1, and OPN; the values are expressed as mean ± standard deviation; *P < 0.05 versus si-NC; #P < 0.05 versus oe-NC

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