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Figure 2 | Journal of Orthopaedic Surgery and Research

Figure 2

From: Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-κB ligand expression in MG-63 osteoblast-like cells exposed to polyethylene particles

Figure 2

RANKL protein levels in MG-63 cells after particle and alpha-CGRP treatment. Time courses of RANKL protein levels in MG-63 osteoblast-like cells stimulated by UHMWPE particles and alpha-CGRP using Western blot analysis. (A) Representative Western blot for RANKL in untreated group and the alpha-CGRP-incubated groups. Densitometric quantification of RANKL in (B) a cell-particle ratio of 1:100 and (C) a cell-particle ratio of 1:500 with and without alpha-CGRP-incubation. RANKL protein levels are expressed relatively to GAPDH. Data are reported as mean ± standard deviation (n = 5). ((a) P < 0.05).

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