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Table 1 Oligonucleotide primer sequences used for real-time quantification RT-PCR detection of type X collagen mRNA

From: Differential expression of type X collagen in a mechanically active 3-D chondrocyte culture system: a quantitative study

Gene

Primer

Sequence

Type X collagen

Forward

5'-AGTGCTGTCATTGATCTCATGGA-3'

 

Reverse

5'-TCAGAGGAATAGAGACCATTGGATT-3'

18S RNA

Forward

5'-CGGCTACCACATCCAAGGAA-3'

 

Reverse

5'-GCTGGAATTACCGCGGCT-3'

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X