Table 1 The sequences of the primers
Name | ACCESSION | Product lengths | Tm | Primer sequence |
|---|---|---|---|---|
iNOS | NM_001313922 | 95 | 60.82 | F:5′-CAGCTGGGCTGTACAAACCTT-3′ |
58.43 | R:5′-CATTGGAAGTGAAGCGTTTCG-3′ | |||
IL-6 | NM_001314054 | 142 | 59.51 | F:5′-CTTCCATCCAGTTGCCTTCTTG-3′ |
59.00 | R:5′-AATTAAGCCTCCGACTTGTGAAG-3′ | |||
Arg-1 | NM_007482 | 249 | 58.37 | F:5′-AACACTCCCCTGACAACCA-3′ |
55.69 | R:5′-CATCACCTTGCCAATCCC-3′ | |||
IL-10 | NM_010548 | 105 | 58.45 | F:5′-GCTCTTACTGACTGGCATGAG-3′ |
60.88 | R:5′-CGCAGCTCTAGGAGCATGTG-3′ | |||
TNF-α | NM_013693 | 164 | 59.31 | F:5′-TCAGTTCCATGGCCCAGAC-3′ |
59.84 | R:5′-GTTGTCTTTGAGATCCATGCCATT-3′ | |||
GAPDH | NM_008084 | 79 | 59.83 | F:5′-CCGCATCTTCTTGTGCAGTG-3′ |
59.42 | R:5′-CGATACGGCCAAATCCGTTC-3′ |