Fig. 6

MiR-331-3p overexpression impeded the malignant phenotypes of MM cells partly by reducing the expression of IGF1R. A–L Cells were transfected with miR-NC, miR-331-3p, miR-331-3p + pcDNA, and miR-331-3p + IGF1R. A Western blot assay was carried out for IGF1R level detection. B, C Cell proliferation was determined by CCK-8 assay and EdU staining. D–F Cell apoptosis and cell cycle were monitored via flow cytometry. (G-H) The protein expression of Bax and Bcl-2 were detected. I–K Corresponding test kits were to assess relative glucose consumption, lactate production and ATP/ADP ratios. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001