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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: Circ_0005615 restrains the progression of multiple myeloma through modulating miR-331-3p and IGF1R regulatory cascade

Fig. 4

The regulation of circ_0005615 downregulation on MM cell progression could be rescued by miR-331-3p inhibitor. MM1.S and NCI-H929 cells were transfected with si-NC, si-circ_0005615, si-circ_0005615 + anti-miR-NC or si-circ_0005615 + anti-miR-331-3p. A QRT-PCR was applied for miR-331-3p expression detection. B, C CCK-8 assay and EdU staining were exploited to measure cell proliferation. DF Flow cytometry was implemented to estimate cell apoptosis and cell cycle. G, H The protein levels of Bax and Bcl-2 were monitored using western blot analysis. IK Glucose consumption, lactate production and the ATP/ADP ratios were calculated to evaluate cell glycolysis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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