Table 1 Primers used for real-time PCR
From: MiR-217 regulates autophagy through OPG/RANKL/RANK in giant cell tumors
| Â | Primer sequences | |
|---|---|---|
miR-217 | (F) | 5′-AACAGTGTACTGCATCAGGAAC -3′ |
(R) | 5′-GTCGTATCCAGTGCAGGGT -3′ | |
U6 | (F) | 5′-GTGCTCGCTTCGGCAGCACATATAC -3′ |
(R) | 5′-AAAAATATGGAACGCTTCACGAATTTG -3′ | |
OCN | (F) | 5′-TGCAGAGTCCAGCAAAGG -3′ |
(R) | 5′-CCCAGCCATTGATACAGGTAG -3′ | |
RUNX2 | (F) | 5′-ACCTTGACCATAACCGTC TTC -3′ |
(R) | 5′-GCTTCTGTCTGTGCCTTCT -3′ | |
OPG | (F) | 5′-CATTCTTCAGGTTTGCTGTTCC -3′ |
(R) | 5′-CTCTCTACACTCTCTGCGTTTAC -3′ | |
RANK | (F) | 5′-CCATGTACCAGTGAGAAGCATTA -3′ |
(R) | 5′-CTGTCAGAGGTAGTAGTGCATTTAG -3′ | |
RANKL | (F) | 5′-GTGGATGGCTCATGGTTAGAT -3′ |
(R) | 5′-GAGGACAGACTCACTTTATGGG -3′ | |
ATG5 | (F) | 5′-CTTCTGCACTGTCCATCTAAGG -3′ |
(R) | 5′-ATCCAGAGTTGCTTGTGATCTT -3′ | |
ATG7 | (F) | 5′-TTGCCCACAGCATCATCTT -3′ |
(R) | 5′-ATGCCTCCTTTCTGGTTCTTT -3′ | |
Beclin-1 | (F) | 5′-GCAGCTGGATAAGCTGAAGA -3′ |
(R) | 5′-CGACCCAGCCTGAAGTTATT -3′ | |
LC3 | (F) | 5′-GTCCTGGACAAGACCAAGTTT -3′ |
(R) | 5′-GTTCACCAGCAGGAAGAAGG -3′ | |
GAPDH | (F) | 5′-CAGGGCTGCTTTTAACTCTGGTAA -3′ |
(R) | 5′-GGGTGGAATCATATTGGAACATGT -3′ | |