Fig. 4

ZFAS1 directedly targeted miR-7-5p. A Subcellular ZFAS1 distribution was analyzed by isolating nuclear and cytoplasmic RNA fractions from chondrocytes, U6 and GAPDH were used as controls. B RNA-FISH was performed to verify the localization of ZFAS1 in the cytoplasm. C Predicted ZFAS1 binding sites with miR-7-5p. D Determination of miR-7-5p expression in chondrocytes transfected with miR-NC or miR-mimics. **P < 0.01, compared with the miR-NC group. E Dual-luciferase reported assay was applied to verify the targeting relationship between ZFAS1 and miR-7-5p. **P < 0.01, compared with the miR-NC group. F RIP with anti-Ago2 and anti-IgG antibodies were performed to analyze endogenous Ago2 binding to RNA. **P < 0.01, compared with the anti-IgG group. G Detection of miR-7-5p expression in chondrocytes with or without IL-1β treatment. **P < 0.01, compared with the control group. H Overexpression of ZFAS1 could suppress miR-7-5p expression in chondrocytes. **P < 0.01, compared with the vector group