Fig. 6

Role of JAK/STAT3 pathway in osteogenesis. A Immunofluorescence staining for STAT3 and p-STAT3 in BMSCs cultured with osteogenic inducing medium with or without Stattic for 7 days. B–D The relative mRNA expression levels of ALPL, RUNX2, BMP2 measured by qRT-PCR. E Immunofluorescence staining for BGLAP. F The protein expression of P-STAT3 and STAT3 in BMSCs cultured with osteogenic inducing medium with or without Stattic for 7 days was detected by WB. G ALP staining (Scale bars = 500 μm). Mean ± SD, n = 3; *p < 0.05, **p < 0.01, ***p < 0.001, ^##p < 0.01, ^###p < 0.001