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Fig. 5 | Journal of Orthopaedic Surgery and Research

Fig. 5

From: Temporal transcriptome highlights the involvement of cytokine/JAK/STAT3 signaling pathway in the osteoinduction of BMSCs

Fig. 5

Gene expression verification by qRT-PCR and immunofluorescence staining. AI The relative mRNA expression level of IL6, LIF, CSF3, RUNX2, BMP2, COL1A1, BGLAP, ACKR3 and VEGFD after 0, 7, 14 and 21 days of inducing (means ± SD, n = 3; *p < 0.05, **p < 0.01, ***p < 0.001). J Immunofluorescence staining for IL6 in BMSCs cultured with osteogenic inducing medium for 7 days. Scale bars = 50 μm

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