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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: Aspirin prevents estrogen deficiency-induced bone loss by inhibiting osteoclastogenesis and promoting osteogenesis

Fig. 4

Aspirin promoted osteogenic differentiation in vitro. A Representative images of ALP staining treated with various concentrations of aspirin. B Aspirin treatment rescued the impaired ALP activity induced by TNF-α. C Representative images of Alizarin Red staining treated with different concentrations of aspirin. D Aspirin treatment reserved the decreased calcified deposits induced by TNF-α. E–H Quantitative analyses of ALP and Alizarin Red staining. **P < 0.01, ***P < 0.001

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