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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Aspirin prevents estrogen deficiency-induced bone loss by inhibiting osteoclastogenesis and promoting osteogenesis

Fig. 3

Aspirin attenuated RANKL-induced NF-κB and MAPK signaling pathways activation. A The NF-κB and MAPK protein levels were examined with western blotting. B The localization of NF-κB p65 subunit was represented with immunofluorescence assay. Scale bar = 20 μm. C The effect of aspirin on the expression of osteoclastic protein, including NFATc1, c-Fos and TRAP. GAPDH was used as an internal control

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