Fig. 3

Over-expression of YAP1 alleviated ICMT-induced endplate chondrocytes degeneration and senescence. A, B Flow cytometry was used to detect the efficiency of YAP1 transfection. C, D Western blotting was used to detect the difference in COL-2A, Sox-9, YAP1, CTGF, p53, and p21 protein expression between each group. E RT-qPCR was used to detect the difference of COL-2A, Sox-9, YAP1, CTGF, p53, and p21protein expression between each group. F–H SA-β-gal staining and F-actin were used to detect the senescence of endplate endplate chondrocytes in each group. I, J Cell cycle and cell Counting Kit-8 were used to observe the effect on the proliferation of endplate endplate chondrocytes in the different groups