Fig. 2

ICMT promotes endplate cartilages degeneration and senescence. A, B Toluidine bluestaining and F-actin confirmed that Ctrl, ICMT 8%,12%, and 16% endplate chondrocytes had different pathological changes in morphology. C, D, F SA-β-gal staining and cell cycle used to detect senescence of endplate chondrocytesin each group. E The effect of different tension of ICMT on the proliferation of endplate chondrocytes. G, H Western blotting was used to detect the difference in COL-2A, Sox-9, YAP1, CTGF, p53, and p21 protein expression between each group. I RT-qPCR was used to detect the difference of COL-2A, Sox-9, YAP1, CTGF, p53, and p21protein expression between each group