Table 1 Primer sequences of GAPDH, PI3K, AKT, and mTOR genes
Gene | Primer | Sequence (5'-3') | Number of bases |
|---|---|---|---|
GAPDH | Forward | ACTCTCCACAGTCAGACCCA | 20 |
| Â | Reverse | ACTCTCCACAGTCAGACCCA | 20 |
PI3K | Forward | GGAATGTCGGGAGCAGCAACC | 21 |
| Â | Reverse | TCTACCACTACGGAGCAGGCATAG | 24 |
AKT | Forward | TCAGGATGTGGATCAGCGAGAGTC | 24 |
| Â | Reverse | AGGCAGCGGATGATAAAGGTGTTA | 24 |
mTOR | Forward | ACCGTCCGCCTTCACAGATACC | 22 |
| Â | Reverse | GCAGTCCGTTCCTTCTCCTTCTTG | 24 |