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Fig. 1 | Journal of Orthopaedic Surgery and Research

Fig. 1

From: Anemonin reduces hydrogen peroxide-induced oxidative stress, inflammation and extracellular matrix degradation in nucleus pulposus cells by regulating NOX4/NF-κB signaling pathway

Fig. 1

ANE repressed the cytotoxic induced by H2O2 in NPCs. A The chemical formula of ANE is shown. B Immunofluorescence detection of aggrecan and collagen II was performed in isolated cells from IVDD tissues. C After treatment with ANE for 48 h, cell viability was measured by MTT assay. D After treatment with H2O2 for 24 h, cell viability was detected by MTT assay. E After treatment with or without ANE for 24 h, the cells were treated with or without H2O2 for 24 h, and then the cell viability was assessed. *p < 0.05 versus control group, **p < 0.01 versus control group

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