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Fig. 5 | Journal of Orthopaedic Surgery and Research

Fig. 5

From: Bone marrow-derived dedifferentiated fat cells exhibit similar phenotype as bone marrow mesenchymal stem cells with high osteogenic differentiation and bone regeneration ability

Fig. 5

Comparison of gene expression changes in SC-DFATs, ASCs, BM-DFATs, and BM-MSCs. SC-DFATs, ASCs, BM-DFATs, and BM-MSCs were cultured in osteogenic or adipogenic differentiation medium for 2 weeks. Total RNA was extracted at indicated time periods, and real-time RT-PCR analysis was performed. A-C Expression of adipogenic marker genes after adipogenic differentiation culture. Expressions of PPARG (A), CEBPA (B), and SLC2A4 (C) were evaluated. D–F Expression of osteogenic marker genes after osteogenic differentiation culture. Expressions of RUNX2 (D), ALPL (E), and BGLAP (F) were evaluated. *p < 0.05 (one-way ANOVA, Tukey’s multiple comparison test)

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