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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: Specificity protein 1-mediated ACSL4 transcription promoted the osteoarthritis progression through suppressing the ferroptosis of chondrocytes

Fig. 4

Acsl4 knockdown neutralized the ferroptosis progression in the IL-1β-treated HCCs. Transfection efficiency of si-Acsl4 was analyzed by RT-qPCR (A). After IL-1β and si-Acsl4 treatment in the HCCs, the LDH release B was measured with LDH Toxicology Kit. Cell viability C was detected by CCK-8 assay. The ROS (D), MDA (E), GSH (F), Fe2+G levels were assessed with Corresponding kits

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