Table 1 List of primers employed in RT-PCR
Name | Primer | Sequence | Size |
|---|---|---|---|
Homo GAPDH | Forward | 5′- TCAAGAAGGTGGTGAAGCAGG -3′ | 115 bp |
|  | Reverse | 5′- TCAAAGGTGGAGGAGTGGGT -3′ |  |
Homo SIRT3 | Forward | 5’- CTTACTAGAGTGCGGCGGT-3’ | 220 bp |
|  | Reverse | 5’- ACAGGTCCACTCATCTTCGT-3’ |  |
U6 | Forward | 5 ‘- CGCTTCGGCAGCACATATAC -3’ |  |
|  | Reverse | 5 ‘- AAATATGGAACGCTTCACGA -3’ |  |
hsa-miR-494 | Forward | 5 ‘-TGCGCAGGTTGTCCGTGTTGTCT-3 ‘ |  |
|  | Reverse | 5′- CCAGTGCAGGGTCCGAGGTATT-3′ |  |