Fig. 7

DHJSD attenuates the IL-1β-induced apoptosis of NPCs by miR-494/SIRT3/mitophagy signal axis. NP cells were untreated, treated with IL-1β (10 ng/mL) alone, or with IL-1β (10 ng/mL) and DHJSD (300 µg/mL). a MiR-494 expression of NP cells treated above was measured using qRT-PCR and ISH separately (scale bar: 25 μm, original magnification ×400). b SIRT3 expression of NPCs treated above was measured using qRT-PCR and WB separately. NPCs were untreated, treated with IL-1β (10 ng/mL) alone, with IL-1β (10 ng/mL) and DHJSD (300 µg/mL), or with IL-1β (10 ng/mL) and DHJSD (300 µg/mL) and mimic control, miR-494 mimic, siScr or si-sirt3. c The protein content of Bcl-2 and Bax and the ratio of cleaved caspase-3/caspase-3 of NPCs treated above. d Representative WB assay and quantitation of Cyt-c content in cytoplasmic and mitochondrial extracts. e Representative fluorescence images with TUNEL staining and quantitative analyses of TUNEL-positive cells (scale bar: 50 μm, original magnification ×200). f The protein content of Parkin, PINK1, LC3-II and p62 of NPCs treated above. g The representative images of mitophagy in NPCs treated above were detected through the mitophagy detection kit, in which red staining denotes mitophagy, green denotes lysosomes, and yellow denotes co-localization of lysosomes and mitophagy (original magnification ×1000, scale bar: 10 μm). All experiments were performed three times in duplicate, and data are shown as average ± SD (n = 3). *P < 0.05 vs. the IL-1β + DHJSD + mimic control. #P < 0.05 vs. the IL-1β + DHJSD + siScr group