Fig. 3

DHJSD attenuates the IL-1β-induced apoptosis of NPCs by promoting mitophagy. NP cells were untreated, treated with IL-1β (10 ng/mL) alone, with IL-1β (10 ng/mL) and DHJSD (300 µg/mL), or with cyclosporin A (1 μM), IL-1β (10 ng/mL) and DHJSD (300 µg/mL). a The protein content of Parkin, PINK1, LC3-II and P62 of NPCs treated above. b The protein content of Bcl-2 and Bax and the ratio of cleaved caspase-3/caspase-3 of NPCs treated above. c Representative WB assay and quantitation of the Cyt-c content in cytoplasmic and mitochondrial extracts. d Representative fluorescence images with TUNEL staining and quantitative analyses of TUNEL-positive cells (scale bar: 50 μm, original magnification ×200). All experiments were performed three times in duplicate, and data are shown as average ± SD (n = 3). *P < 0.05 vs. the control. #P < 0.05 vs. the IL-1β group. & < 0.05 vs. the DHJSD + IL-1β group