Fig. 5

SLIT2 mediated the pro-osteogenic function of miR-382 in hBMSC-sEV. A CCK8 assays and B qRT-PCR of miR-382 and SLIT2 expression in MG63 cells treated with PBS or hBMSC-sEV after transfection of negative control or SLIT2 over-expression plasmid. C ALP staining, D alizarin red S staining and E western blot analysis of SLIT2 and osteogenesis-associated markers in MG63 cells treated with PBS or hBMSC-sEV following transfection of negative control or SLIT2 over-expression plasmid after osteogenic differentiation. Statistical evaluation was performed using two-way ANOVA. Mean ± SEM. *represents P < 0.05 compared with NC group. *P < 0.05, ***P < 0.001; # represents P < 0.05 compared with HG group. ###P < 0.001; &represents P < 0.05 compared with miR-182-5p group. &&P < 0.01, &&&P < 0.001