Table 1 Primer sequences for qPCR
From: MiR-760 targets HBEGF to control cartilage extracellular matrix degradation in osteoarthritis
Gene | Primers |
|---|---|
MiR-760 | Forward: UUCUCCGAACGUGUCACGUTT Reverse: ACGUGACACGUUCGGAGAATT |
MMP3 | Forward: AGTCTTCCAATCCTACTGTTGCT Reverse: TCCCCGTCACCTCCAATCC |
MMP13 | Forward: ACTGAGAGGCTCCGAGAAATG Reverse: GAACCCCGCATCTTGGCTT |
ADAMTS4 | Forward: GAGGAGGAGATCGTGTTTCCA Reverse: CCAGCTCTAGTAGCAGCGTC |
COL2A1 | Forward: TGGACGATCAGGCGAAACC Reverse: GCTGCGGATGCTCTCAATCT |
Aggrecan | Forward: ACTCTGGGTTTTCGTGACTCT Reverse: ACACTCAGCGAGTTGTCATGG |
HBEGF | Forward: ATCGTGGGGCTTCTCATGTTT Reverse: TTAGTCATGCCCAACTTCACTTT |
CBL | Forward: TGGTGCGGTTGTGTCAGAAC Reverse: GGTAGGTATCTGGTAGCAGGTC |
CAMK2G | Forward: ACCCGTTTCACCGACGACTA Reverse: CTCCTGCGTGGAGGTTTTCTT |
MAP2K1 | Forward: CAATGGCGGTGTGGTGTTC Reverse: GATTGCGGGTTTGATCTCCAG |
ADCY1 | Forward: AGGCACGACAATGTGAGCATC Reverse: TTCATCGAACTTGCCGAAGAG |
RPS6KA3 | Forward: CGCTGAGAATGGACAGCAAAT Reverse: TCCAAATGATCCCTGCCCTAAT |
U6 | Forward: CTCGCTTCGGCAGCACA Reverse: AACGCTTCACGAATTTGCGT |
β-actin | Forward: AGATGTGGATCAGCAAGCAG Reverse: GCGCAAGTTAGGTTTTGTCA |