Fig. 1From: MiR-760 targets HBEGF to control cartilage extracellular matrix degradation in osteoarthritisDegenerative OA-associated cartilage exhibited higher levels of miR-760 expression than nondegenerative cartilage. A Schematic overview of the classification of degenerative and nondegenerative cartilage tissue samples. B Representative safranin-O/fast green staining of cartilage from control or OA patients. Scale bar: 100 µm. C MiR-760 expression levels in OA and non-OA cartilage samples. n = 20 (twenty different donors). Student's t test (t test) was used for comparisons between two groups. *p < 0.05. D, E The expression of miR-760 in chondrocytes stimulated with TNF-α (10 ng/mL) and IL-1β (10 ng/mL) for 24 h, 48 h, and 72 h. n = 3 (1 technical replicate on 3 different donors). Data were analysed by one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test for comparison between the control and treatment groups. *, p < 0.05 compared with 0 h. MiR-760 regulates chondrocyte-mediated degradation of the extracellular matrix. F, I After 24 h of transfection, the efficiency of the overexpression or knockdown of miR-760 in chondrocytes transfected with the indicated constructs. n = 3 with 3 technical replicates. Student's t test (t test) was used for comparisons between two groups. G, J After 24 h, cartilage metabolism-related gene expression was analysed in chondrocytes via qPCR following the overexpression or inhibition of miR-760. n = 3 with 3 technical replicates. Student's t test (t test) was used for comparisons between two groups. *p < 0.05. H, K After 48 h, cartilage metabolism-related protein expression was examined in chondrocytes following the overexpression or inhibition of miR-760 via Western immunoblotting. n = 3; three different donors. miRNAs, microRNAs; qPCR, real-time quantitative PCR. OA, osteoarthritis; TNF, tumour necrosis factor; IL, interleukinBack to article page