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Table 1 Primers’ Sequences Used in the Real-Time PCR (5'—3')

From: IL-1β contributes to the secretion of sclerostin by osteocytes and targeting sclerostin promotes spinal fusion at early stages

Name

Forward

Reverse

GAPDH

AAGGTCGGTGTGAACGGATT

TGAGTGGAGTCATACTGGAACAT

Sclerositn

CGTGCCTCATCTGCCTACTTGT

CGGTTCATGGTCTGGTTGTTCT

ALP

ACGGCGTCCATGAGCAGAACTA

CAGGCACAGTGGTCAAGGTTGG

OCN

CCAAGCAGGAGGGCAATAAGGT

CTCGTCACAAGCAGGGTTAAGC

OPN

AGCAAGAAACTCTTCCAAGCAA

GTGAGATTCGTCAGATTCATCCG

Runx2

CCCAGGCAGTTCCCAAGCATTT

GGTAGTGAGTGGTGGCGGACAT

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X