Skip to main content
Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: IL-1β contributes to the secretion of sclerostin by osteocytes and targeting sclerostin promotes spinal fusion at early stages

Fig. 4

Alizarin red S staining and ALP staining of MC3T3-E1 cells. A Representative images of ALP staining. Scale bar, 200 µm. B Representative photographs of ALP stained wells. C Quantification of ALP positive area normalised to the control group. D Representative images of mineralised nodules stained with Alizarin red S. Scale bar, 200 µm. E Representative photographs of ARS stained wells. F Quantification of calcium deposition normalised to the control group. The experiments in vitro were performed independently 3 times. *p < 0.05 and **p < 0.01. n.s., not significant

Back to article page